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01 奈良県立医科大学 >
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01121 Journal of Nara Medical Association >
Vol.40 No.2 >
このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10564/2292
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タイトル: | 2種の異種抗体を用いたELISAによるα₂-アンチプラスミン抗原量の測定 |
その他のタイトル: | α₂-ANTIPLASMIN ANTIGEN ASSAY BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) USING TWO KINDS OF POLYCLONAL ANTIBODIES |
著者: | 松岡, 宏明 橋本, 憲治 金廣, 照美 福井, 弘 高瀬, 俊夫 杉本, 充彦 三上, 貞昭 |
キーワード: | 異種抗体 Alpha-2-Antiplasmin ELISA enzyme-linked immunosorbent assay (ELISA) α₂-antiplasmin (α₂-AP) α₂-AP antigen α₂-AP activity amidolytic assay |
発行日: | 1989年4月30日 |
出版者: | 奈良医学会 |
引用: | 奈良医学雑誌 Vol.40 No.2 p.264-270 |
抄録: | A new solid enzyme-linked immunosorbent assay (ELISA) for human α₂-antiplasmin (α₂-AP) antigen was developed using two kinds of anti-human α₂-AP antiserum. The procedure of α₂-AP antigen assay is as follows : Step 1 ; a microtiter plate is coated with an anti-human α₂-AP guineapig antiserum for 24 hrs at 4℃. Step 2 ; serially diluted plasma is added to the plate and incubated for 24 hrs at 4℃. Step 3 ; after washing with a phosphate buffered saline, an anti-human α₂-AP rabbit antiserum is added to the plate and incubated for 3 hrs at 37℃. Then, a peroxidase conjugated anti-rabbit IgG goat antiserum is added and incubated for 1 hr at 37℃. After the 3rd step, the microtiter plate is washed extensively. Finally, the α₂-AP antigen is determined by ELISA method using o-phenylendiamin as the substrate. The lower limit of α₂-AP antigen in this assay was 0.1 U/dl. In 25 healthy adult males and 25 adult females, the mean values of α₂-AP were 96.8±12.2 U/dl and 95.2±10.7 U/dl, respectively. A good correlation was found between the α₂-AP level determined by this ELISA method and by Laurell's method. There was also a good correlation between α₂-AP activity by an amidolytic method and antigen level by the ELISA. |
URI: | http://hdl.handle.net/10564/2292 |
ISSN: | 04695550 13450069 |
出現コレクション: | Vol.40 No.2
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