ANGIOTENSIN Ⅱ TYPE Ⅰ RECEPTOR AND ANGIOTENSINOGEN GENE POLYMORPHISMS IN PATIENTS WITH CORONARY ARTERY DISEASE

Abstract

To analyze the participation of genetic factors in the onset and process of coronary arteriosclerosis, we investigated the distribution of both angiotensin Ⅱ type Ⅰ receptor (AT1R) and angiotensinogen (ATN) gene polymorphisms, and the relationship between genotype and clinical features in patients with coronary artery disease (CAD). We studied 139 CAD patients (CAD group : 93 males, 46 females, mean age of 62 y/o) consisting of 118 with myocardial infarction and 21 with angina pectoris. We selected 133 healthy volunteers without overt cardiac heart disease as control subjects. Al166C polymorphism of the AT1R gene (A → C transversion at position 1166 of AT1R gene) and T174M polymorphism of ATN gene (T → M transversion at position 174 of ATN gene) were determined by PCR of genomic DNA extracted from peripheral leukocytes and digestion with restriction enzyme. Patients were classified into one of three genotypes, AA homozygote, AC heterozygote and CC homozygote for AT1R, TT, TM and MM for ATN. The distribution of AT1R and ATN genotype did not differ between CAD patients and control subjects. Neither AT1R nor ATN genotype was correlated with the severity of coronary arteriosclerosis. In AT1R gene polymorphism, AC and CC genotype were significantly more frequent in patients with than in those without restenosis. In ATN gene polymorphism, TT genotype was also significantly more frequent in patients with than in those without restenosis. In conclusion, both ATIR and ATN gene polymorphism may participate in the process of restenosis after coronary angioplasty.